Investigation of metabolic family genes for the clinical nipple tumefaction proteomes

Investigation of metabolic family genes for the clinical nipple tumefaction proteomes Cells were grown in glutamine and glucose-free RPMI, supplemented either with 2 mmol/L 13 C5 or 15 N2-glutamine or 5 mmol/L 13 Csix– d -glucose (Cambridge Isotope Laboratories), 10% dialyzed FBS, and 1% antibiotics (penicillin–streptomycin). After 24-hour growth, metabolites were extracted with methanol: acetonitrile: […]